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Researcher Job in Physical chemistry – Fluorescence microscopy techniques

Lund University

Sweden

Lund University, Faculty of Science, Department of Chemistry recruits Researcher in physical chemistry with Project title: Bacterial interactions of antimicrobial peptides characterized using advanced fluorescence microscopy – relation between peptide binding and bacterial destabilization – Sweden, Oct 2021.

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General Info

Position: Researcher
No. of Positions: 1
Research Field:
Deadline to Apply: Expired
Joining Date: -
Contract Period: Not Mentioned
Salary: According to Standard Norms

Workplace:
Faculty of Science
Department of Chemistry
Lund University
Lund,

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Qualification Details

Applicants must have:

  • A PhD or equivalent research qualification within the subject of the position.
  • Very good oral and written proficiency in English.

Assessment criteria and other qualificationsPrevious experience of the fluorescence microscopy techniques mentioned above is a merit. Previous experience of working with AMPs is a merit. Previous experience of working with bacteria is a merit. Documented ability of conducting efficient and high-qualitative research, independent as well as in a group, is a merit.

Consideration will also be given to good collaborative skills, drive and independence, and how the applicant’s experience and skills complement and strengthen ongoing research within the department, and how they stand to contribute to its future development.

Responsibilities/Job Description

The main task of the project is to characterize the interaction of AMPs with bacteria (particularly Escherichia coli) using a set of advanced fluorescence microscopy techniques. This includes in particular:

  • Using single-molecule and super-resolution microscopy to study where on the bacterial cell the AMPs bind.
  • Study of fluorescently labelled AMPs regarding bacteria binding/uptake investigating location, kinetics, and cooperativity.
  • Correlating the above to the distribution of anionic phospholipids (particularly cardiolipin) within the bacterial membrane.
  • Diffusion of lipids or of bound peptides within bacterial membranes, measured with fluorescence recovery after photobleaching or single molecule tracking, and its dependence on bound AMP.
  • Monitoring effects of AMP binding on aggregation and re-distribution of bacterial lipopolysaccharide (LPS) by anti-LPS antibodies.
  • Consequences of these processes on bacterial membrane damage monitored by transport of membrane-impermeable dyes into/out of the bacteria.

How to Apply?

Online Application through "Apply Now" Button from this page or from advertisement webpage (URL below)

Reference Number: -PA2021/2882
(If any, use it in the necessary place)

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Documents Required

Applications shall be written in English and be compiled into a PDF-file containing:

  • résumé/CV, including a list of publications,
  • a general description of past research and future research interests (no more than three pages),
  • contact information of at least two references,
  • copy of the doctoral degree certificate, and other certificates/grades that you wish to be considered.

About the Project

The purpose of the project is to employ advanced fluorescence microscopy for the investigation of interactions of antimicrobial peptides (AMPs) with bacteria. In doing so, the project aims to clarify the interplay between bacterial membrane composition and AMP binding, as well as consequences of such binding for compositional and structural changes in bacterial membranes, eventually leading to bacterial lysis. As such, the project is expected to provide a novel perspective to the mode-of-action of AMPs, bringing together previous progress in the action of AMPs in model lipid membranes, on one hand, and biological results on antimicrobial effects, on the other. The project will be conducted at the Division of Physical Chemistry, Lund University, under the supervision of Prof. Martin Malmsten https://pharmacy.ku.dk/employees/?pure=en/persons/566760 and Assoc. Prof. Peter Jönsson https://www.physchem.lu.se/people/seniors/joensson/ in collaboration with researchers in the groups of Malmsten and Jönsson at both Copenhagen University and Lund University. 

About the Employer: Lund University

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Contact details

  • Peter Jönsson, Assoc. professor, peter.jonsson@fkem1.lu.se
  • Martin Malmsten, professor, martin.malmsten@sund.ku.dk
  • Magdalena Brossing, HR coordinator, +46 46 222 95 62

Advertisement Details: Researcher in physical chemistry

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