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Research Engineer for Clinical Proteomics Mass Spectrometry – Karolinska Institutet, Sweden


We are now looking for a PhD-level research engineer with experience of core facility work or equivalent to join the team working in the Mass Spectrometry core facility, at Department of Oncology-Pathology, Karolinska Institutet, Sweden – Nov 2021

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General Info

Position: Research Engineer
No. of Positions: 1
Research Field: , , ,
Joining Date: Jan 01, 2022
Contract Period: Permanent position
Salary: According to Standard Norms

Department of Oncology-Pathology
Karolinska Institutet
Karolinska Institute (KI)

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Qualification Details

The successful candidate needs to meet our requirements.


  • PhD degree in analytical chemistry, biochemistry, biotechnology or in a related field
  • Years of experience in core facility work
  • Extensive hands-on experience of laboratory work and ability to follow SOPs
  • Experience with techniques applied to sample preparation for proteomics, including protein extraction, clean-up (e.g. by solid phase extraction), protein concentration measurement, as well as protein depletion and/or equalization methodologies and protein and peptide separation/fractionation using gel-based electrophoresis (1D, 2D and HiRIEF).
  • Hands-on experience with Single-Pot Solid-Phase-enhanced Sample Preparation (SP3) and in gel/in solution protein reduction/alkylation and protein digestion techniques
  • Practical experience in liquid chromatography mass spectrometry (LC-MS)
  • Experience in Mass spectrometry Maintenance and Troubleshooting
  • Experience in quantitative MS-based proteomics (TMT10 plex, TMT16 plex)
  • Good knowledge in methods used for analysis of omics data
  • Excellent communication and organizational skills
  • Good knowledge (both speaking and writing) of English


  • Competence/experience in MS-based clinical proteomics research
  • Competence/experience in cell culture.
  • Competence in sample preparation and mass spectrometry analysis of native peptides and immunopeptidome
  • Collaborative experience with hospitals and clinical sample handling.
  • Data management and data services
  • Strong interests of working in centralized shared research infrastructures

We place great emphasis on personal suitability. To succeed in the role, it is important that the applicant has a personal drive and flexible mind to handle the rapidly developing field of proteomics and the need of custom-based solutions in many projects. The applicant should be responsible, appreciate daily detailed work as well as have the ability to see the big picture. The applicant should be an effective communicator, have good organizational skills, and be able to prioritize at times when the workload is high. The applicant should be able to collaborate with people of different backgrounds and at different levels, both within the group and with users from various disciplines.

Responsibilities/Job Description

The responsibilities accompanying this position include sample preparation, bio analytical work, analysis and reporting of protein/peptide expression, method development, use/optimization of the MS instruments, data analysis and data interpretation. Experiences in method development, data analysis and reporting are important.

The role also includes reporting analysis results to facility users, taking part of undergraduate/postgraduate teaching/training and representing the facilities at national and international meetings and conferences.

The research engineer will be part of a research infrastructure, working closely with project scientists, postdoctoral fellows, PhD-students, undergraduate students, and external users/customers within academia and industry. Important aspects of the job include:

  • Preparation of clinical and in-vitro samples for proteomics
  • Experimental analysis of samples by mass spectrometry
  • Compiling and analyzing proteomics data
  • Continuous contact with customers by email and phone

The work involves a combination of flexible and routine tasks and places a great emphasis on independence, initiative, precision, and planning. The position involves a variety of tasks in a stimulating research environment. Nice and helpful colleagues contribute to a good working environment within the group.

A large part of the job may be contact with customers/researchers from a wide range of fields, which is an exciting, interesting and instructive part of the work. The facility/research group is located at the Science for Life Laboratory with spacious and modern facilities.

We are seeking a highly motivated research engineer to be part of our mass spectrometry core facility.

How to Apply?

Online Application through "Apply Now" Button from this page

Reference Number: STÖD 2-4906/2021
(If any, use it in the necessary place)

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Documents Required

Documents required to complete the online application process

About the Project/Department

At the Department of Oncology-Pathology basic, translational and clinical research and educational activities related to cancer is carried out. Approximately 300 people from over 40 nations are currently working at the department. About 30 research groups with various cancer research profiles are involved and we have around 110 PhD students. The Department of Oncology-Pathology is responsible for undergraduate courses in Pathology, Oncology and Forensic Medicine for medical students, as well as for Tumor biology courses for biomedicine students and Pathology courses for opticians.

The position is within the regional and the national core facilities led by Professor Janne Lehtiö, located at the Science for Life Laboratory in Solna. The regional core facility is tasked with aiding users of Swedish universities and industry with mass spectrometry (MS) based proteomics projects. The national facility (SciLifeLab/BioMS) offers infrastructure for biological mass spectrometry ( Combined, the regional and national facilities offer a unique research infrastructure and expertise with focus on MS-based proteomics and proteogenomics. The core facility works in close collaboration with Prof. Janne Lehtiö’s research group, where MS-based proteomics methods are constantly developed and applied to studies aiming at improving individualized cancer therapy. This collaboration is a prerequisite for the core facility’s ability to offer services at the forefront of MS based proteomics.

Learn more about the Lehtiö lab and Clinical Proteomics Mass Spectrometry Core Facility.

About the Employer: Karolinska Institute (KI)

Note or Other details

Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote better health for all. At Karolinska Institutet, we conduct innovative medical research and provide the largest range of biomedical education in Sweden. Karolinska Institutet is a state university, which entitles employees to several benefits such as extended holiday and a generous occupational pension. Employees also have free access to our modern gym and receive reimbursements for medical care.

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Contact details

  • Helena Bäckvall, 08-524 812 09
  • Lukas Orre, 08-517 763 70

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