Postdoctoral position: How do LPMO enzymes attack crystalline polysaccharide surfaces in the degradation of biomass? A structural investigation by solid-state NMR spectroscopy is available for a master’s degree in chemistry, biophysics, NMR spectroscopy, or structural biology at the Department of Chemistry and Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Denmark 2022
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The applicant is expected to:- be independent, creative and dependable- have good collegial, oral and written communication skills- have proven scientific excellence- hold a PhD degree in chemistry, biophysics or related molecular disciplines- ideally, have prior experience with NMR spectroscopy or structural biology- ideally, have prior experience and/or done course work in biochemistry or molecular biology
Experience with biological solid-state NMR spectroscopy is an advantage. The project will require protein expression and purification skills for sample generation. This part requires a 6 month stay at NTNU in Norway (Trondheim).Minor involvement in teaching activities may be solicited. The place of work is Gustav Wieds Vej 14, 8000 Aarhus C, and the area of employment is Aarhus University with related departments.
The capacity of biofuels to make a significant contribution to sustainability depends on ways to overcome the recalcitrance of abundant and widespread polysaccharides, most notably cellulose. Lytic polysaccharide monooxygenase enzymes (LPMOs) can attack such recalcitrant polysaccharides, improving the efficiency of canonical commercialized mixtures of hydrolytic enzymes to depolymerize biomass efficiently. Important progress awaits molecular structural insight into LPMO action and deactivation. This will be obtained in the proposed project: innovations affording ultrafast spinning now allow solid-state NMR spectroscopy to probe polymeric and crystalline polysaccharide surfaces and provide the structural details of how LPMO proteins are bound to them, breaking a principal barrier.
Project Goals and Tasks
The experimental approach taken is structure determination by solid-state NMR spectroscopy, complemented with molecular docking. For the core NMR project, access to modern solid-state MAS NMR on in-house 700 and 950 MHz spectrometers is available. Isotope-labelled protein samples are generated by the candidate at NTNU in Norway (in the group of Prof. Aachmann).
How to Apply?
Application Method: Online Application
Ref. No.: 8391
The application must be in English and include a curriculum vitae, degree certificate, a complete list of publications, a statement of future research plans and information about research activities, teaching portfolio and verified information on previous teaching experience (if any). Guidelines for applicants can be found here.
About the Department/Section/Group
The Ultra High Field NMR Center at Aarhus University offers a stimulating research environment, covering complementary research activities in the area of liquid state NMR, oriented and MAS solid state NMR, HR-MAS, pulse sequence development, low-field NMR, high-pressure NMR and imaging. The lab is equipped with state-of-the-art spectrometers operating at 950 MHz, 700 MHz, 500 MHz, and 400 MHz. The BioNMR research is embedded within the Department of Chemistry (https://chem.au.dk/en/) and the Interdisciplinary Nanoscience Center (iNANO; http://inano.au.dk). These provide abundant and state-of-the-art techniques in structural and molecular biology as well as a vibrant environment with multiple keen NMR research groups.Aarhus University further provides a strong international research and training environment with excellent professional and private conditions. Foreign employees can get help and information from the AU International Center (http://www.au.dk/en/internationalcentre/) and Work in Denmark (https://workindenmark.dk/). Tax reduction schemes for researchers may apply. For information about our laboratory, activities and recent publications please follow these links: Link to the Danish Center for Ultrahigh Field NMR Spectroscopy
Note or Other details
Shortlisting is used. This means that after the deadline for applications – and with the assistance from the assessment committee chairman, and the appointment committee if necessary, – the head of department selects the candidates to be evaluated. All applicants will be notified whether or not their applications have been sent to an expert assessment committee for evaluation. The selected applicants will be informed about the composition of the committee, and each applicant is given the opportunity to comment on the part of the assessment that concerns him/her self. Once the recruitment process is completed a final letter of rejection is sent to the deselected applicants.
Letter of reference
If you want a referee to upload a letter of reference on your behalf, please state the referee’s contact information when you submit your application. We strongly recommend that you make an agreement with the person in question before you enter the referee’s contact information, and that you ensure that the referee has enough time to write the letter of reference before the application deadline. Unfortunately, it is not possible to ensure that letters of reference received after the application deadline will be taken into consideration.
Formalities and salary range
Natural Sciences refers to the Ministerial Order on the Appointment of Academic Staff at Danish Universities under the Danish Ministry of Science, Technology and Innovation. Appointment shall be in accordance with the collective labour agreement between the Danish Ministry of Finance and the Danish Confederation of Professional Associations. Further information on qualification requirements and job content may be found in the Memorandum on Job Structure for Academic Staff at Danish Universities. Salary depends on seniority as agreed between the Danish Ministry of Finance and the Confederation of Professional Associations. Aarhus University’s ambition is to be an attractive and inspiring workplace for all and to foster a culture in which each individual has opportunities to thrive, achieve and develop. We view equality and diversity as assets, and we welcome all applicants.Research activities will be evaluated in relation to actual research time. Thus, we encourage applicants to specify periods of leave without research activities, in order to be able to subtract these periods from the span of the scientific career during the evaluation of scientific productivity.Aarhus University offers a broad variety of services for international researchers and accompanying families, including relocation service and career counselling to expat partners. Read more here. Please find more information about entering and working in Denmark here.Aarhus University also offers a Junior Researcher Development Programme targeted at career development for postdocs at AU. You can read more about it here.The application must be submitted via Aarhus University’s recruitment system, which can be accessed under the job advertisement on Aarhus University's website.
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Applicants seeking further information are invited to contact: Prof. Dr. Frans A.A. Mulder Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University Phone +45 51 44 73 44Email [email protected]nmr.au.dk // inano.au.dk
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