Assistant Professor in CRISPR-mediated Gene Editing Combined with Proteomics to Study the Function, Structure, Regulation, and Targeting of Nuclear Exosome Adaptor Complexes in RNA Decay

The Centre for Exo-Adapt invites applications from outstanding candidates who are interested in joining our team as an assistant professor. The candidate will be affiliated with the Jens S. Andersen group at the Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense. The position is available for three years from Marts 2023 or as soon as possible thereafter. Application deadline: 27 February 2023.

The RNA Exosome

Our genome produces RNA that either gives rise to protein or to independent RNA molecules. However, genomic DNA is hyperactive, and only a fraction of the RNA produced ends up as functional molecules – the rest is degraded. The exosome complexes are the molecular machines that detect and remove undesirable RNA so that cells do not drown in their own molecular waste. The core complex of the exosome has been comprehensively studied, revealing that it is merely a general RNA degradation machine. Instead, specific recognition of its multitude of substrates is achieved by the association of the exosome with so-called protein adaptor complexes. Although some of these have been identified, we do not understand how they recognize and discriminate their substrates, how they interact with the exosome and how these interactions are regulated to achieve adequate RNA turnover at steady state or in dynamic cellular settings.

Exo-Adapt

The Centre for Exo-Adapt is a collaborative project supported by a grant from the Novo Nordisk Foundation Challenge Programme. The Exo-Adapt initiative aims to study the function, structure, regulation, and targeting of nuclear exosome adaptor complexes with a role in RNA degradation using a combination of genomics, proteomics, and cryo-electron microscopy approaches.The project involves the following PIs:

  • Torben Heick Jensen (coordinator), Department of Molecular Biology and Genetics, University of Aarhus, Denmark
  • Elena Conti, Max Planck Institute of Biochemistry, Munich, Germany
  • Jens S. Andersen, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.

Research Project and Role

As an assistant professor in Exo-Adapt you will be involved in highly ambitious and interdisciplinary projects covering functional and structural studies of protein-RNA complexes using for example CRISPR-mediated gene editing for tagging of proteins for protein-protein interaction and protein proximity labelling analysis, fluorescence microscopy, protein and PTM analysis by mass spectrometry-based proteomics, and characterization of RNA substrates by RNA sequencing and bioinformatic approaches.

You will interact and collaborate closely with other researchers in the Jens S. Andersen, Torben Heick Jensen, and Elena Conti groups as well as with members of the other research groups at the Biomedical Mass Spectrometry and Systems Biology Section at the Department of Biochemistry and Molecular Biology.

We offer:

  • A research position available for three years in a stimulating and supportive, international research environment.
  • Access to state-of-the-art equipment, facilities, and infrastructure within proteomics, including a unique collection of advanced instrumentation for mass spectrometry (Orbitrap Exploris 480 and Orbitrap Eclipse from Thermo Fisher Scientific, and timsTOF Pro and flex from Bruker) and for bioimaging (Abberior facility line STED and MINFLUX, Nikon 3D STORM, and Nikon dual cam SIM).
  • Opportunity to exchange knowledge, discuss project progress, and visit Exo-Adapt partners for cross-laboratory research projects.
  • Opportunity to interact with a wider network of national and international collaborators.
  • Opportunity to develop your own ideas within our research frame.
  • Mentoring and career development.
  • A competitive salary and social benefits (e.g. health coverage, parental leave, social security etc.).
  • Dedicated assistance and support with various practical, social, and legal aspects (housing, visa, administration, tax etc.), especially for international candidates through our international staff office (ISO) and department.

Qualifications/Requirements

We are looking for a highly motivated, enthusiastic, and ambitious candidate with:

  • A PhD degree in biochemistry, biomedicine, molecular biology, protein science, analytical biochemistry, or similar field.
  • Experience in CRISRP-mediated gene editing and/or mass spectrometry-based proteomics for cell biological studies.
  • Proficiency in computational data analysis and statistics.
  • Strong communication and collaborative skills and an interest in working in an international and interdisciplinary environment.
  • An open and positive mind to contribute positively to the success of the Centre for Exo-Adapt and well-being of the research team.
  • Proficiency in spoken and written English.

In addition to research, the applicant must be able to teach and advise students.Applicants will primarily be evaluated based on their documented international scholarly and publication achievements.For further information, please contact Professor Jens S. Andersen (jens.andersen@bmb.sdu.dk).

Application, salary etc.

Appointment to the position will be in accordance with the salary agreement between the Ministry of Finance and the Danish Confederation of Professional Associations. Please check links for more information on salary and taxation.Applications will be assessed by an expert committee. Applicants will be informed of their assessment by the university.

The application must be in English and include the following:

• A curriculum vitae with indications of references

• A full list of publications

• Copies of all publications on which the applicant wishes to be evaluated

• A brief survey of the applicant’s most important research results and research plans for the next three years. Shortlisting may be used in the assessment process. Incomplete applications and applications received after the deadline will neither be considered nor evaluated. Please note that applicants are expected to have completed their PhD. The University wishes our staff to reflect the diversity of society and thus welcomes applications from all qualified candidates regardless of personal background. Applications must be submitted electronically using the link “Apply online”. Attached files must be in Adobe PDF or Word format. Each box can only contain a single file of max. 10 Mb. We strongly recommend that you read How to apply before you apply. Since not all members of the appointment committee are Danish-speaking, it is recommended that your application is submitted in English. Further information for international applicants about entering and working in Denmark.