The Zampieri group offers a post-doctoral research opportunity in the context of systems pharmacology, computational biology and metabolic regulation. This position will be based in the Department of Biomedicine (https://biomedizin.unibas.ch/en/) in Basel, one of the world’s leading locations for life sciences, home to several companies and institutions.The mission of the group is to understand fundamental mechanisms regulating short- and long-term metabolic adaptation to genetic and chemical perturbations to find new and unconventional therapeutic strategies, ranging from antibacterial to anticancer drugs. To this end, we develop new ways to combine state-of-the-art technologies in metabolomics with mathematical modeling.The candidate will have the opportunity to exploit some of the cutting-edge experimental and computational methods, comprising constraint-based and kinetic modeling, statistical analysis of large datasets, high-throughput metabolomics, time-lapse microscopy, to investigate how to pharmacologically interfere with fundamental mechanisms in the regulation of bacterial metabolism. Available resources at the department include a mouse facility, high-end FACS, IT and microscopy facilities, the Life Sciences Training Facility (for gene expression and proteome profiling) and much more.The candidate is expected to have a strong background and interest in quantitative disciplines, excellent teamwork and communication skills in English. The candidate will have the opportunity to develop the following project with a lot of academic freedom and strong support from senior members in the lab, and at the same time to play an active role in shaping and in creating inspiring research and working environment.In line with our and Uni Basel values (https://www.unibas.ch/en/Research/Values-Ethics/Diversity.html), we are committed to sustain and promote an inclusive culture, ensure equal opportunities and value diversity and respect in our working and learning environment.
Project: Study how bacterial susceptibility to antibiotics depends on the metabolic and physiological states of bacteria. In the framework of the NCCR AntiResist (https://www.nccr-antiresist.ch/en/), we are looking for a highly motivated Post-Doc researcher to study how bacterial susceptibility to antibiotics depends on the metabolic and physiological states of bacteria (30% wet lab and 70% computational).
- Apply and develop model-based and statistical approaches to analyze and interpret large compendia of metabolome profiling of small molecules effects on bacteria.
- Adapting existing and developing improved experimental protocols for the high-throughput metabolomics analysis of drug-perturbed bacteria across different growth conditions.
- Unravel the hidden potential of apparently inactive molecules in hampering bacterial infection
- Determining the underlying mechanisms that link antibacterial efficacy to the metabolic or physiological state of bacteria.
- Testing their relevance in host-like environmental conditions
- Support and preparation of scientific reports and journal articles
Your profile Essential:
- Master’s degree and/or PhD in (Bio)Physics, (Bio)engineering, Biological Sciences, Microbiology, Biochemistry, Systems Biology, Molecular Biology, Bioinformatics/Computational Biology, or related fields.
- Basic experience of designing and running experiments with microbial cultures in the laboratory
- Experience with programming language (e.g. Matlab, R or Python)
- Motivation to work in an international research environment
- Hands-on experience with key biochemical and molecular biological methods
- Experience with and/or motivation to learn systems biology approaches, high-throughput methodologies.
- Good communication skills
- Interest in mathematical modeling of metabolic and regulatory/signaling network
We offer you
- You will have access to state-of-the-art research equipment in metabolomics, cell biology, time-lapse microscopy.
- You will work in a dynamic and highly interdisciplinary team including computer scientists, experimentalists and clinicians.
- You will be involved in cross-disciplinary collaborations and have training opportunities to further develop and grow your scientific interests.
- Access to a large consortium of experts in different disciplines, such chemistry, engineering, clinics, computational biology and (Micro- / Cell- / Immune-)Biology (https://www.nccr-antiresist.ch/en/about-us/organization-structure/principal-investigators).
- Opportunity to work in close contact with other basic- and clinical-research groups at the Department of Biomedicine and the Biozentrum in Basel
- Support from highly competent, experienced team members and potential to lead projects with a high degree of academic freedom
- Ortmayr, K., de la Cruz Moreno, R. & Zampieri, M. Expanding the search for small-molecule antibacterials by multidimensional profiling. Nat Chem Biol 18, 584–595 (2022)
- Anglada-Girotto, M., Handschin, G., Ortmayr, K. et al. Combining CRISPRi and metabolomics for functional annotation of compound libraries. Nat Chem Biol 18, 482–491 (2022).
- Zampieri, M., Hörl, M., Hotz, F. et al. Regulatory mechanisms underlying coordination of amino acid and glucose catabolism in Escherichia coli. Nat Commun 10, 3354 (2019)
- Øyås O., Borrell S., Trauner A., et al. Model-based integration of genomics and metabolomics reveals SNP functionality in Mycobacterium tuberculosis. PNAS 117 (2020)
- Campos A. and Zampieri M. Metabolomics-Driven Exploration of the Chemical Drug Space to Predict Combination Antimicrobial Therapies. Molecular Cell 74, 1291-1303 (2020)
Application / Contact Please send your application via the University of Basel Recruiting-Portal https://www.unibas.ch/en/Working-at-the-University-of-Basel/Current-Vacancies.html, by submitting the following requested documents: A) a cover letter, B) a complete curriculum vitae C) names and email addresses of at least two referees.